Coding

Part:BBa_K5184015:Design

Designed by: Ziyan Zhou   Group: iGEM24_GreatBay-SCIE   (2024-09-10)


SltNPPS-Mvan4662-ShZIS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1.Optimization of all codons for E. coli based on E. coli codon bias. 2.Removal of N-terminus anchoring region to avoid incompatibility between the membrane recognition signal of heterogeneous proteins and the eukaryotic host. 3.ShZIS is put right behind the promoter, thus ensuring its highest expression level among the three.


Source

Solanum Lycopersium, Mycobacterium vanbaalenii, Solanum habrochaites

References